Keywords

• lowest price Proteinase K powder
• good supplier 39450-01-6
• High quality Proteinase K

Quick Details

• ProName: lowest price Proteinase K powder good ...
• CasNo: 39450-01-6
• Molecular Formula: C29H27N2O12P
• Appearance: White powder
• Application: Health care grade,API,
• DeliveryTime: 5-7 working days
• PackAge: Bottle, Drum, Vacuum Packed, Plastic b...
• Port: China major port
• ProductionCapacity: 55 Metric Ton/Month
• Purity: 99.5%
• Storage: Sealed, dry and refrigerated
• Transportation: By Express,Air,Sea
• LimitNum: 1 Gram
• Active Ingredient: Proteinase K
• Specification: 99.5%
• Einecs No.: 254-457-8
• Application: Health care grade，API
• Sample: 2g
• MOQ: 1G
• Place of Origin: Shaanxi，China
• Brand Name: QUANAO
• Other name: Carbonicacid

Superiority

lowest price Proteinase K powder good supplier 39450-01-6 High quality Proteinase K

Proteinase K [EC 3.4.21.64] is a stable and highly reactive serine protease enzyme belonging to the subtilisin family. Proteinase K [EC 3.4.21.64] contains two disulfide bridges and one free Cys near the active site His. Proteinase K [EC 3.4.21.64] has an active-site catalytic triad (Asp39-His69-Ser224). Proteinase K [EC 3.4.21.64] hydrolyzes peptide amides. Proteinase K [EC 3.4.21.64] catalyzes the reaction of keratin hydrolysis, as well as other proteins with substilisin-like specificity. Originally sourced from the mold Tritirachium album Limber, Proteinase K [EC 3.4.21.64] is also formerly known as the previous designations EC 3.4.4.16 and EC 3.4.21.14.

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca ²⁺. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

GoldBio's Proteinase K undergoes two sterile filtration steps during the separation and purification processes. The final product is further analyzed by plate culture to ensure the removal of all living organisms was 100% effective.

Details

Proteinase K Typical Properties

Item
Specifications
Results

Appearance (Color)
White to Off-White
White

Appearance (Form)
Powder
Powder

Protein by Biuret
≥ 95 %
97.9 %

Solubility (Turbidity)1mg/ml, H2O
Clear
Clear

activity
≥35 units/mg
38 units/mg

Store at
-20°C

Conclusion
The results conforms with Enterprise standards

Proteinase K Usage
Inactivation of nuclease protein during DNA and RNA isolation.
Removal of the restriction of cationic protein by endotoxin and digestion of protein in brain tissue samples for the study of prion infectious spongiform encephalopathy (TSE)
Proteinase K Packaging and Shipping
Packaging： 100g/bag，  25kgs/drum, inner by double plastic bag, 1~20kg by aluminum foil vacuum bag.
Shipping: By air, by sea or by courier
Proteinase KStorage
50 Kilogram/Kilograms per Month